WebApr 25, 2012 · All washes were performed using a Bio-Plex Pro wash station. Data acquisition was performed using Bio-Plex Manager™ 6.0 at a low PMT setting. The t … WebThis panel allows simultaneous quantification of 13 mouse cytokines, including IL-1α, IL-1β, IL-3, IL-7, IL-11, IL-12p40, IL-12p70, IL-23, IL-27, IL-33, IFN-β, GM-CSF and TSLP. This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected ...
Bio-Plex Pro Mouse Cytokine 23-plex Assay #M60009RDPD
WebPrepare a 25-fold working dilution of the anti-cytokine bead (25x) stock solution in Bio-Plex Assay Buffer A Document all volume calculations. Protect the beads from light. Keep all bead solutions on ice when not in use. ASSAY Cover all unneeded wells with plastic adhesive plate sealer. Set the vacuum pressure to 2 inches Hg http://web.mit.edu/dallab/downloads/Cytokine_BioPlex_Short_Protocol.doc speedghost ab
Bio-Plex Pro Mouse Cytokine 23-plex Assay #M60009RDPD
WebHuman Single Bio-Plex Pro™ Cell Signaling Bead Kits and Bio-Plex Pro™ Magnetic Cell Signaling Buffer Assay Kit (all Bio-Rad, USA) were used to detect quantitative changes in total protein concentration, as well as phosphorylated levels of ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), JNK (Thr183/Tyr185), p65 subunit of NF-κB (Ser536 ... WebFeb 1, 2024 · Quantitation of 31 cytokines in mouse serum was determined using a Bio-Plex Pro™ Mouse Chemokine Panel 31-Plex (Bio-Rad, Hercules, CA, USA) as described [26]. The assay was performed using mouse ... WebMar 30, 2024 · Bio-Plex Pro™ Rat Cytokine Th1/Th2 Assay #171K1002M (Analytes: GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, and TNF-α) were used to evaluate the immunological changes in the presence of LIP. The entire colon tissue was first measured with a plastic ruler, excised in two equal sections, and weighed on an ... speedghost