Phospho-akt thr308
WebPrimary antibody: Akt phospho T308 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min ... View more Product Details Product Specific Information Store vial at 4° C prior to restoration. WebThe Phospho-Akt (Thr308) Antibody was generated using AKT1, AKT2, and AKT3 as the antigen and it reacts with Human, Mouse, and Rat. Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308 Phospho-Akt (Thr308) Antibody Supplier Page Product Specs Company Info Citations Reviews Product Specs
Phospho-akt thr308
Did you know?
WebThe Phospho-AKT (Thr308) kit is designed for robust quantification of AKT modulation, phosphorylated on Thr308 across all isoforms (AKT1/2/3). Also known as protein kinase B (PKB), AKT is an oncogene playing a key role in controlling apoptosis, cell proliferation, transcription, cell migration, and glucose metabolism. WebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins.
WebMar 22, 2024 · The protein levels of p-Akt (S473 and T308), Akt, p-rpS6 (S235/236, S240/244), rpS6, FLAG and β-actin were detected with immunoblotting. e HEK293T cells were transfected with FLAG empty vector or FLAG-DRAM1 for 24 h, and then starved for another 18 h before stimulated with IGF-1, EGF or serum (10%) for 10 min in the presence … WebInvitrogen Anti-Phospho-AKT1 (Thr308) Polyclonal, Catalog # 44-602G. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, …
WebAkt/PKB growth Ser241 Thr308 Ser473 Akt/PKB Thr308 Ser473 glycogen synthesis proliferation survival PI3K Akt, Akt Section 03-02-06 PIP 3 PIP 3 Tyr376 Tyr373 SHIP 05_PW_Akt/PKB.eps Tyr326 Src M e m b r a n e PDK1 P P P P P P P P P P P P P P P P mTOR SIN1 GβL Rictor Akt-PKB KSignaling Pathway Phospho-Akt (Ser473) kDa 105 76 57 46.5 ... WebThis Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308). Key Applications: Immunohistochemistry (Paraffin) Applications …
WebAKT phosphorylation sites of Ser473 and Thr308 regulate AKT degradation. Protein kinase B (AKT) is a serine-threonine kinase that mediates diverse cellular processes in a variety …
WebApr 5, 2024 · Akt activation can lead to the phosphorylation of RXRXX(S/T) sequences of transcription factors, controlling apoptosis or pro-survival molecules [10]. For instance, PI3K/Akt activation by NTFs mediates in vitro MN survival, whereas their inhibition causes apoptotic cell death [11, 12]. birch haven resortWebMore>> Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA. Less<< MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. dallas elementary schools txWebThis Anti-phospho-Akt (Thr308) Antibody is validated for use in IH (P) for the detection of phospho-Akt (Thr308). Anti-phospho-Akt (Thr308) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA birch headboardWebMar 3, 2024 · Phospho-Akt (p-Akt) on Ser473 (S473), p-Akt on Thr308 (T308), and Akt protein expression in cancerous tissues and survival analysis of ovarian cancer patients were directly obtained from The Cancer Proteome Atlas (TCPA) database 1 … birch haywards heathWebJan 22, 2009 · AML patients were stratified into two groups, Thr308 high or Thr308 low, according to the value of phospho-Akt Thr308 (rMFI >2.3, Thr308 high and rMFI ⩽ 2.3, Thr308 low). birch haven resort boveydallas electricity for apartmentsAkt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor ... dallas employment services reviews