Phospho stat3 flow cytometry
WebMOLT4 cells are characterized by higher basal STAT3 phosphorylation. These data document that phospho-specific flow cytometry reveals a high variability in intrinsic as well as modulated signaling networks across different T-ALL cell lines. WebFlow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to the …
Phospho stat3 flow cytometry
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WebMar 14, 2013 · Additionally, STAT3 has a prolonged activation over other STATs and plays a critical role in the IL-21 signaling . pSTAT3 measurements resulted in a very good dynamic range for flow cytometry analysis allowing the generation of excellent dose response curves and a good IC 50 determinaion. Thus, phospho-STAT3 was selected as a biomarker for IL … WebJan 1, 2012 · Flow cytometry can be used to study STAT phosphorylation on a per cell basis. Cells are fixed, permeablized, and stained with antibodies that specifically bind to …
WebPhospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) detects endogenous levels of Stat3 only when phosphorylated at Tyr705. This antibody does not cross-react with phospho-EGFR or the … WebJul 3, 2014 · Phospho-flow detection of constitutive and cytokine-induced pSTAT3/5, pAKT and pERK expression highlights novel prognostic biomarkers for patients with multiple …
WebPhospho Targets Need a control for your phospho flow cytometry staining? Our Veri-Cells™ Phospho PBMCs can be used as a positive and negative control for staining members of …
WebFlow cytometry quantification of phospho-STAT3, phospho-STAT5 and expression of Bcl-2/Bcl-XL after TQ treatment. Phosphorylation of STAT3 (A) and STAT5 (B) as well as the …
WebOct 3, 2024 · Flow cytometry analysis was performed on BD FACSCanto II cytometer. Data analysis was performed with FCS Express 6 software. The target cells were gated on FSC/SSC plot to remove debris, followed by a singlet gate on FSC-H/FSCW plot. ... SKBr3 cancer cell lines probed for total and phospho-STAT3 by Western blotting. (B) … bistro chicken twist pampered chefWebJul 31, 2007 · Flow cytometry of simultaneous phosphorylated NF-κB and STAT3 expression from B-cells, T-lymphocytes and monocytes/macrophages stimulated by IL1β (control). Cytogram (A and C) depicted one experiment and showed the isotype control (A1 and C1 for B-cells, A4 and C4 for T-lymphocytes and A7 and C7 for monocytes/macrophages). dartmouth hitchcock medical center phoneWebApr 1, 2012 · The expression of STAT3, pSTAT3, Smad 2/3, phospho-Smad3, ... of LFs isolated from different compartments of the non-IPF lungs for Thy-1/CD90 cell surface … dartmouth hitchcock medical center obgynWebNov 15, 2013 · In order to validate a phospho-specific flow cytometry assay assessing phospho-signal transducer and activator of transcription factor 5 (p-STAT5) as a new diagnostic tool for JMML, we examined 22 samples from children with JMML and 47 controls. CD33+/CD34+ cells from 22 patients with JMML showed hyperphosphorylation … dartmouth hitchcock medical center urologyWebDec 21, 2012 · Flow Cytometry. BTSC spheres were dissociated to single cells, ... Millipore), phospho-STAT3 Y705 (1:50; Cell Signaling Technology), phospho-STAT3 S727 (1:50; Cell Signaling Technology), human nucleolin (1:1000; Millipore), and human nuclear antigen (1:20; Chemicon). Staining was visualized with Vectastain Elite mouse immunoglobulin … bistro chinese osawaWebHuman Phospho-STAT3 (S727) Antibody Summary Species Reactivity Human Specificity Detects human STAT3 when phosphorylated at S727. Source Monoclonal Mouse IgG 2B Clone # 788335 Purification Protein A or G purified from hybridoma culture supernatant Immunogen Phosphopeptide containing the human STAT3 S727 site Formulation bistro chick roppongiWebImmunogen: Phosphorylated Human Stat3 Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645327 Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage bistro chick 六本木